P-796 Single-cell transcriptomics unveils a role for leptin receptor in human endometrial mesenchymal stromal/stem cells

Abstract Study question What are the characteristics of leptin receptor (LepR) expressing cells within endometrial mesenchymal stromal/stem (eMSCs) population? Summary answer LepR+ eMSCs display better stem-cell capacities as well distinctively quiescent properties compared to and LepR- eMSCs. is known already Single-cell RNA sequencing (scRNA-seq) reveals that cultured heterogeneous. LepR highly expressed in bone marrow MSCs, contribute towards formation fibroblast colony-forming units (CFU-F). Mouse MSCs activate upon injuries form osteoblasts adipocytes marrow. In this study, we hypothesized play a critical role stem cell population human endometrium. design, size, duration We performed scRNA-seq on magnetic bead selected (CD140b+CD146+ cells). Functional assays such clonogenicity serial subcloning were different stromal subsets (eMSC, eMSC, eMSC) isolated by fluorescence-activated sorting (FACS) cells. The cycle analysis was evaluated multicolor flow cytometry freshly Participants/materials, setting, methods samples collected from women aged 37–53 years with regular menstrual cycles undergoing hysterectomy. expression localization endometrium determined immunohistochemistry, respectively. vitro self-renewal abilities assessed FACS sorted populations. status property qPCR (RB1, RBL2, CDKN1A, CDKN1B, E2F4). Main results chance Clustering data revealed 5 subpopulations (SP0-4) A high proportion (>90%) SP3 SP4 at G1 phase. majority (92%) located root trajectory pseudo time inference analysis. specific suggesting potentially origin progenitors.The 2.61 ± 1.43%, 25.59 19.04%. experiments demonstrated exhibit properties, including clonogenic ability, than its negative counterparts. QPCR validation cell-quiescence associated genes higher RB1, CDKN1B addition, significantly G0 phase when above indicate vivo relatively more quiescent. Limitations, reasons for caution mechanism involved maintaining or activating remains unknown. likely be mouse. also lack an model studying during repair. Wider implications findings Our extend understanding heterogeneous unveil new regeneration. Trial registration number not applicatble